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PubMed · 3203144

Practical hints for peptide sequencing by soft ionization methods.

W Kausler·K Schneider·G Spiteller
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Abstract

Practical hints are summarized for the investigation of peptides by soft ionization methods based on liquid secondary ion mass spectrometry. The method aims: to purify the sample; to force a peptide to the surface by ion pair formation; to recover the sample used for mass spectral analysis; to distinguish between Lys and Gln by derivatization; to spot amino acids in the low mass region; and to improve sequence information by use of peptide fragments, not described previously.

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Amino AcidsChromatography, High Pressure LiquidMass SpectrometryPeptide FragmentsPeptide MappingPeptidesJournal ArticleResearch Support, Non-U.S. Gov't

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W Kausler, K Schneider, G Spiteller. 1988. Practical hints for peptide sequencing by soft ionization methods.. https://doi.org/10.1002/bms.1200170106

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