Engineering antibody affinity and specificity.

D M Webster; S Roberts; J C Cheetham; R Griest; A R Rees

doi:10.1002/ijc.2910410804

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Engineering antibody affinity and specificity.

D M Webster·S Roberts·J C Cheetham·R Griest·A R Rees

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Abstract

A combination of ab initio calculations, "knowledge-based prediction", molecular graphics and site-directed mutagenesis has enabled us to probe the molecular details of antibody:antigen recognition and binding and to alter the affinity and specificity of an antibody for its antigen. The significance of electrostatic hydrogen bonding, hydrophilic/hydrophobic patch matching and van der Waals interactions as well as CDR:CDR interactions are discussed in relation to the results of site-directed mutagenesis experiments on the anti-lysozyme antibody Gloop2. The ability to generate reconstructed antibodies, chimeric antibodies, catalytic antibodies and the use of modelled antibodies for the design of drugs is discussed.

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Animals Antibody Affinity Antibody Specificity Humans Hydrogen Bonding Mice Models, Molecular Muramidase Mutation Protein Engineering Rats Journal Article

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D M Webster, S Roberts, J C Cheetham, R Griest, A R Rees. 1988. Engineering antibody affinity and specificity.. https://doi.org/10.1002/ijc.2910410804

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