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Calcium-binding lysozymes.

K Nitta·H Tsuge·K Shimazaki·S Sugai
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Abstract

It was found that pigeon lysozyme binds one calcium ion, as does equine lysozyme. The protein was eluted with equimolar calcium ions from a Bio-Gel P-60 column. The binding constants of equine and pigeon lysozymes were determined to be 2 x 10(6) and 1.6 x 10(7) M-1, respectively, in 0.1 M KCl at pH 7.1 and 20 degrees C. During evolution the gene of calcium-binding lysozyme is deduced to be separated from that of non-calcium-binding lysozyme by gene duplication before splitting of avian and mammalian lineages, from their amino-acid sequences. It is assumed that the alpha-lactalbumin might have evolved from calcium-binding lysozyme.

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AnimalsCalciumCalcium-Binding ProteinsChromatography, GelColumbidaeEgg WhiteFemaleKineticsMuramidaseSpecies SpecificityJournal Article

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K Nitta, H Tsuge, K Shimazaki, S Sugai. 1988. Calcium-binding lysozymes.. https://doi.org/10.1515/bchm3.1988.369.2.671

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