Improved methods for separation and purification by affinity chromatography.

G Johnson; J S Garvey

doi:10.1016/0022-1759(77)90014-x

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Improved methods for separation and purification by affinity chromatography.

G Johnson·J S Garvey

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Abstract

Defined conditions for simplified Affinity Chromatography suitable for instructional and research purposes, were developed. The standard method of activation of polysaccharide gels using BrCN was modified and improved using a highly buffered reaction solution. A batchwise protocol for the immunoadsorbent isolation of anti-BSA was used (with commonly employed dissociation agents) and comparative data were obtained.

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Acetonitriles Animals Antibodies Chromatography, Affinity Cyanogen Bromide Goats Immunosorbent Techniques Serum Albumin, Bovine Journal Article

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G Johnson, J S Garvey. 1977. Improved methods for separation and purification by affinity chromatography.. https://doi.org/10.1016/0022-1759(77)90014-x

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