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Optical errors in a liquid chromatography absorbance cell.

K Peck·M D Morris
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Abstract

The shadowgraph method is used to visualize mobile phase flow through a liquid chromatography absorbance cell with "Z" flow geometry. It is shown that mixing is incomplete in the cell. Composition gradients persist throughout the elution of any sample. The gradients systematically change as the sample flows through the cell. Additionally, window wedge angles are measured in several cells. The implications for low noise absorbance detectors are discussed.

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Azo CompoundsChromatography, High Pressure LiquidChromatography, LiquidIndicators and ReagentsRefractometryJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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K Peck, M D Morris. 1988-09-02. Optical errors in a liquid chromatography absorbance cell.. https://doi.org/10.1016/s0021-9673(01)84582-9

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