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PubMed · 323273

A continuous-flow enzyme detector for liquid chromatography.

T D Schlabach·S H Chang·K M Gooding·F E Regnier
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Abstract

A detection system has been developed for the selective and sensitive detection of enzymes eluting from a liquid chromatographic column. This system monitors a reaction that the enzyme catalyzes and provides a chemical amplification ranging from 10(4) to 10(5). The detection system consists of a reagent or substrate pump, a post-column reactor packed with non-porous spherical glass beads, and a photometric detector. A linear and selective response to a series of enzymes of clinical importance is demonstrated.

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Alcohol OxidoreductasesAlkaline PhosphataseAutoanalysisChromatography, High Pressure LiquidDithionitrobenzoic AcidEnzymesIndicators and ReagentsKineticsL-Lactate DehydrogenaseMathematicsMicrochemistrySaccharomyces cerevisiaeSpectrophotometry, UltravioletTrypsinJournal Article

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T D Schlabach, S H Chang, K M Gooding, F E Regnier. 1977-04-01. A continuous-flow enzyme detector for liquid chromatography.. https://doi.org/10.1016/s0021-9673(00)82573-x

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