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Thiol exchange catalysed refolding of small proteins utilizing solid-phase supports.

D C Smith·R C Hider
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Abstract

The study of isolated snake toxin refolding has been a valuable tool in the understanding of protein folding dynamics. We report here differences in the refolding characteristics of three toxin classes and introduce a novel method for overcoming disulphide mismatching and oligomer formation by utilizing solid-phase thiol exchange gels.

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Chromatography, High Pressure LiquidCircular DichroismElapid VenomsElectrophoresis, Polyacrylamide GelIsoelectric FocusingKineticsProtein ConformationSulfhydryl CompoundsJournal Article

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D C Smith, R C Hider. 1988. Thiol exchange catalysed refolding of small proteins utilizing solid-phase supports.. https://doi.org/10.1016/0301-4622(88)80004-8

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