PubMed · 32364
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R Segal, E Azaz, M Donbrow. 1979. Peroxide removal from non-ionic surfactants.. https://doi.org/10.1111/j.2042-7158.1979.tb13418.x
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The precursor of an RNA molecule from T4-infected E. coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions [(UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. We studied the self-cleavage reaction of an RNA fragment (GUUUCGUACAAAC) (R1) with the sequence corresponding to the p2Sp1 RNA in the presence of Mg2+ and non-ionic detergents. It requires Mg2+ and is aided by a non-ionic detergent, Brij 58. The cleavage reaction is time, temperature, and pH-dependent. The cleavage occurs at the phosphodiester bond between UpA and CpA on the RNA fragment (GUUUCGUACAAAC) (R1). Furthermore, the maximum of cleavage of R1 occurs at a very low Mg2+ concentration (< or = 5 mM).
The sedimentation volume, redispersibility and appearance of a polystyrene latex suspension in the presence of nonionic surface active agent of polyoxyethylene glycol monoethers of n-hexadecanol have been investigated. At low concentrations of nonionic surface agents, hard caked suspensions were found. This is probably due to the attraction between the uncoated areas or the mutual adsorption of the adsorbed molecules on the bare surface of the particles in the sediment. At high concentrations of nonionic surface active agents, the redispersibility of the particles was affected by the steric repulsive force.
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