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PubMed · 3256722

A novel method for nucleic acid sequence determination.

W Bains·G C Smith
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Abstract

We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.

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Base SequenceMethodsNucleic Acid HybridizationRepetitive Sequences, Nucleic AcidJournal Article

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W Bains, G C Smith. 1988-12-07. A novel method for nucleic acid sequence determination.. https://doi.org/10.1016/s0022-5193(88)80246-7

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