High-efficiency generation of plasmid cDNA libraries using electro-transformation.

E C Böttger

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PubMed · 3273198

High-efficiency generation of plasmid cDNA libraries using electro-transformation.

E C Böttger

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Abstract

A simple and efficient method for the construction of large cDNA libraries in plasmid vectors is described. Cloning efficiencies of greater than 1 x 10(6) recombinants per microgram starting RNA are easily obtained using electroporation. A detailed protocol for determining the optimal conditions using this novel approach is presented.

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Base Sequence Calcium Phosphates Chemical Precipitation DNA Electrodes Gene Library Humans Molecular Sequence Data Plasmids Transformation, Genetic Journal Article Research Support, Non-U.S. Gov't

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E C Böttger. 1988. High-efficiency generation of plasmid cDNA libraries using electro-transformation.. https://pubmed.ncbi.nlm.nih.gov/3273198/

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