Electron microscopy study of Q beta replicase.

N H Berestowskaya; V D Vasiliev; A A Volkov; A B Chetverin

doi:10.1016/0014-5793(88)80012-7

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Electron microscopy study of Q beta replicase.

N H Berestowskaya·V D Vasiliev·A A Volkov·A B Chetverin

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Abstract

Purified preparations of Q beta replicase have been studied by electron microscopy using a negative staining technique, and a three-dimensional model of the enzyme molecule has been constructed. The molecule of this four-subunit protein appears to be a compact structure having a size of 100 +/- 10 A; it is subdivided into two unequal bipartite subparticles. The conclusion has been made that all the constituent subunits, including the ribosomal protein Sl, acquire a globular conformation when associated in the replicase complex.

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Coliphages Escherichia coli Microscopy, Electron Protein Conformation Q beta Replicase RNA Nucleotidyltransferases Journal Article

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N H Berestowskaya, V D Vasiliev, A A Volkov, A B Chetverin. 1988-02-15. Electron microscopy study of Q beta replicase.. https://doi.org/10.1016/0014-5793(88)80012-7

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