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PubMed · 3287708

A modified assay for cyclosporin in blood using solid-phase extraction with high-performance liquid chromatography.

B G Charles·R L Norris·P J Ravenscroft
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Abstract

A modified procedure for measuring cyclosporin in whole blood by high-performance liquid chromatography is described and evaluated for clinical use. Sample preparation uses solid-phase extraction cartridges that can be reused. Life of the reverse-phase analytical column exceeds 1,000 injections at 70 degrees C. Cyclosporins A and D (internal standard) elute after 5.6 and 7.6 min, respectively. Calibration plots are linear from 50 ng/ml to at least 2,000 ng/ml. Within-day and between-day imprecision is less than 9% (coefficient of variation). Minimum measurable concentration is 50 ng/ml.

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Chromatography, High Pressure LiquidClinical Trials as TopicCyclosporinsHumansMonitoring, PhysiologicClinical TrialJournal Article

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B G Charles, R L Norris, P J Ravenscroft. 1988. A modified assay for cyclosporin in blood using solid-phase extraction with high-performance liquid chromatography.. https://pubmed.ncbi.nlm.nih.gov/3287708/

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