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PubMed · 3291983

Asynchronous antigen expression in B lineage acute lymphoblastic leukemia.

C A Hurwitz·M R Loken·M L Graham·J E Karp·M J Borowitz·D J Pullen·C I Civin
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Abstract

Cell surface phenotypes of 113 B lineage acute lymphocytic leukemia (ALL) cases, defined by the presence of HLA-DR and at least one B-cell-specific antigen (either CD19, CD20, or CD22), were compared with antigen-defined stages of normal B lymphocyte development. The cases were first evaluated for expression of HLA-DR, CD19, CD34, CD10, CD20, and CD22 by indirect one-color immunofluorescence. Pairwise comparisons of cell surface marker expression were performed for each leukemic sample: no correlations were observed for paired antigen expression on the leukemic samples using antigens expressed either early or late during normal B lymphoid development. Complete immunophenotypes of the cases were then compared with normal B-cell developmental stages. Sixteen different complete immunophenotypes were observed on the leukemias that were not found in normal marrow; at least 78% of the cases demonstrated such "asynchronous" combinations of B lymphoid-associated differentiation antigens. Several samples were subsequently studied by two-color immunofluorescence, and the presence of doubly labeled cells with "asynchronous" antigen combinations was confirmed. These results indicate that the majority of B lineage leukemias exhibit "developmental asynchrony," as compared with normal marrow B cells. The data further suggest that ALL cases do not accurately represent cells arrested at the stage where the leukemogenic event occurred. Rather, ALL appears to be a disease in which there may be maturation of leukemic blasts; but this maturation is "asynchronous" when compared with the normal developmental process.

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AdultAntigens, Differentiation, B-LymphocyteAntigens, NeoplasmB-LymphocytesCell DifferentiationChildFluorescent Antibody TechniqueHumansLeukemia, LymphoidPhenotypeJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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C A Hurwitz, M R Loken, M L Graham, J E Karp, M J Borowitz, D J Pullen, C I Civin. 1988. Asynchronous antigen expression in B lineage acute lymphoblastic leukemia.. https://pubmed.ncbi.nlm.nih.gov/3291983/

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