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Immunoelectron microscopic study of glomerular lesions using a postembedding method with a protein A-gold complex.

M Nakajima·T Hirota·K Kusumoto·K Taira·H Kamitsuji
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Abstract

Renal biopsy tissue from 33 children with various glomerular diseases has been investigated by electron microscopy using a postembedding immunostaining technique with a protein A-gold complex in order to establish more precise correlations between immunopathologic and morphologic findings in glomeruli. This technique could detect immunoglobulins (IgG, IgA, and IgM), complement factor (C3c), and fibrinogen-related antigen. The immunoreactivity of these antigens was essentially confined to the mesangial, paramesangial, subendothelial, and subepithelial 'electron-dense deposits' in the glomeruli. Except for IgM and C3c in the case of glomerular sclerosis, the distribution of the mentioned factors was even in the electron-dense deposits, as could be shown by 'double-immunolabeling'. From the above-mentioned findings one can conclude that several of the localized factors are associated with the formation of electron-dense deposits, the ultrastructural hallmarks of glomerular disease.

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AntigensComplement C3Complement C3cGoldHumansImmunoglobulinsImmunologic TechniquesKidney DiseasesKidney GlomerulusMicroscopy, ElectronMicroscopy, FluorescenceStaphylococcal Protein AJournal Article

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M Nakajima, T Hirota, K Kusumoto, K Taira, H Kamitsuji. 1987. Immunoelectron microscopic study of glomerular lesions using a postembedding method with a protein A-gold complex.. https://doi.org/10.1159/000184338

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