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PubMed · 3301002

A yeast operator overlaps an upstream activation site.

J W Kronstad·J A Holly·V L MacKay
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Abstract

The product of the BAR1 gene of Saccharomyces cerevisiae is synthesized only in the a cell type and inactivates alpha-factor, the mating pheromone made by alpha cells. The MAT alpha 2 protein represses the transcription of a-cell-specific genes, including BAR1, in alpha and a/alpha diploid cells. Transcription of BAR1 in a cells in stimulated upon exposure to alpha-factor. Deletion analysis of the 5' noncoding region of the BAR1 gene revealed that the major upstream activation site (UAS) overlaps the 31 bp operator sequence required for MAT alpha 2 repression. This result has implications for the negative control of transcription in yeast. The deletion analysis also indicated that the sequence TGAAACA mediates alpha-factor stimulation.

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Base SequenceGene Expression RegulationGenes, FungalMutationOperator Regions, GeneticPheromonesRepressor ProteinsSaccharomyces cerevisiaeTranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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J W Kronstad, J A Holly, V L MacKay. 1987-07-31. A yeast operator overlaps an upstream activation site.. https://doi.org/10.1016/0092-8674(87)90491-0

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