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PubMed · 33129

Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet.

J S Tse·Y Shibayama·B J Lin
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Abstract

With a cytochemical method using guanylyl imidodiphosphate as a substrate, the guanylate cyclase activity was localized on the plasma membrane of A, B and D cells of islets of Langerhans isolated from the rat. Adequate control experiments were performed by a double-blind method. Parallel biochemical assay showed that guanylate cyclase activity was not completely lost after fixation with 1% glutaraldehyde and incubation with 4 mM lead nitrate. Furthermore, the depressed activity was still stimulatable with acetylcholine.

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AcetylcholineAnimalsCell MembraneGuanylate CyclaseGuanylyl ImidodiphosphateHistocytochemistryIslets of LangerhansMaleRatsJournal Article

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J S Tse, Y Shibayama, B J Lin. 1978-12-13. Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet.. https://doi.org/10.1007/bf00495386

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