Extracellular iron chelation in Cryptococcus neoformans.

E S Jacobson; M J Petro

Home/Archive/PubMed

PubMed · 3325632

Extracellular iron chelation in Cryptococcus neoformans.

E S Jacobson·M J Petro

View original source ↗Export metadata ↓

Abstract

Low-iron minimal medium supported growth of Cryptococcus neoformans but spent medium contained no hydroxamates, organic acids or other iron chelators. Exogenous deferoxamine stimulated growth in extreme iron-limitation, while neither organic acids nor quartersaturated transferrins were stimulatory. These results suggest two iron uptake mechanisms, one dependent upon exogenous hydroxamate, the other hydroxamate-independent.

Explore related subjects

Cryptococcus Cryptococcus neoformans Culture Media Hydrogen-Ion Concentration Iron Chelating Agents Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

E S Jacobson, M J Petro. 1987. Extracellular iron chelation in Cryptococcus neoformans.. https://pubmed.ncbi.nlm.nih.gov/3325632/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Cryptococcus zeae, a new yeast species associated with Zea mays.

A new yeast, Cryptococcus zeae (type strain HB 1207(T)) is described. Six strains were isolated from corn and pests of corn in Austria. Microsatellite-primed polymerase chain reaction (MSP-PCR) fingerprints showed that the strains are members of the same species. Phylogenetical analyses of domains D1/D2 26S rDNA and ITS 1-5,8S-ITS 2 sequences showed C. zeae to have the closest relationship to C. luteolus. The D1/D2 sequences of C. zeae fit with three Korean Cryptococcus sp. strains (AF459690, AF459691, AF459692). The new species is separable from the closest relative C. luteolus using only two physiological tests.

Cryptococcus↗

PubMed2006

Cryptococcus stepposus, a new filobasidiaceous yeast species found in the Prioksko-terrasny biosphere reserve in Russia.

Mycocinotyping of cryptococci from the Prioksko-terrasny reserve (Russia) has revealed three strains that have a unique mycocin-sensitivity profile. Sequencing results of the D1/D2 domain of the 26S rDNA and of the complete ITS region placed them in the Floriforme clade of the Filobasidiales lineage (Hymenomycetes). The three strains had identical sequences, which differed from those of known Filobasidium and Cryptococcus species. A novel species named Cryptococcus stepposus (type strain VKM Y-2918) is proposed to accommodate these isolates. Physiological characteristics distinguishing the novel species from other Cryptococcus species in the Floriforme clade are presented.

Cryptococcus↗

PubMed2005

Interspecies differences in the enantioselectivity of epoxide hydrolases in Cryptococcus laurentii (Kufferath) C.E. Skinner and Cryptococcus podzolicus (Bab'jeva & Reshetova) Golubev.

Isolates representing Cryptococcus laurentii and Cryptococcus podzolicus, originating from soil of a heathland indigenous to South Africa, were screened for the presence of enantioselective epoxide hydrolases for 2,2-disubstituted epoxides. Epoxide hydrolase activity for the 2,2-disubstituted epoxide (+/-)-2-methyl-2-pentyl oxirane was found to be abundantly present in all isolates. The stereochemistry of the products formed by the epoxide hydrolase enzymes from isolates belonging to the two species (11 isolates representing C. laurentii and 23 isolates representing C. podzolicus) was investigated. The enantiopreferences of the epoxide hydrolases for 2,2-disubstituted epoxides of these two species were found to be opposite. All strains of C. laurentii preferentially hydrolysed the (S)-epoxides while all C. podzolicus isolates preferentially hydrolysed the (R)-epoxides of (+/-)-2,2-disubstituted epoxides. These findings indicate that the stereochemistry of the products formed from 2,2-disubstituted epoxides by the epoxide hydrolase enzymes of these yeasts should be evaluated as additional taxonomic criterion within the genus Cryptococcus. Also, the selectivity of some epoxide hydrolases originating from isolates of C. podzolicus was high enough to be considered for application in biotransformations for the synthesis of enantiopure epoxides and vicinal diols.

Cryptococcus↗