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PubMed · 3325697

Retinal wholemounts: a simple method for precise mapping.

P P Giorgi·M L Graydon
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Abstract

A simple and inexpensive method to analyse retinal wholemounts is described. It allows the definition of regularly spaced sites to map regional specializations of the retina under light microscopy. After attaching a piece of translucent millimetre graph paper behind the slide holding the preparation, the two grid patterns of the graph paper and of the eye-piece grid are used in combination for the definition of sites for analysis and the return to specific areas as small as 50-100 microns2. The present method is simpler and offers several advantages when compared to others based on X-Y movements of the microscope stage or on photographic montage.

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Evaluation Studies as TopicHistological TechniquesRetinaRetinal Ganglion CellsJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

P P Giorgi, M L Graydon. 1987. Retinal wholemounts: a simple method for precise mapping.. https://doi.org/10.1016/0165-0270(87)90005-7

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