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Rapid screening method for enterotoxigenic Escherichia coli.

M Gurwith
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Abstract

A rapid screening method for detection of Escherichia coli producing heat-labile enterotoxin is described. Single colonies are transferred directly from primary culture plates into 96-well microculture plates containing 0.3 ml of brain heart infusion broth in each well. After 24 h at 37 degrees C, each brain heart infusion broth culture is assayed by the miniculture method in the corresponding well of a microculture plate in which Y-1 mouse adrenal tumor cells have been grown. All enterotoxigenic isolates detected by this method were confirmed in the assay but with culture supernatants.

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Adrenal Gland NeoplasmsAdultAnimalsBacteriological TechniquesCell LineChildCulture MediaEnterotoxinsEscherichia coliFecesHumansMiceTemperatureComparative StudyJournal Article

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BibTeXRIS

M Gurwith. 1977. Rapid screening method for enterotoxigenic Escherichia coli.. https://doi.org/10.1128/jcm.6.3.314-316.1977

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