Selective decrease in low-Mr HMG proteins HMG I and HMG Y during differentiation of mouse teratocarcinoma cells.

E Vartiainen; J Palvimo; A Mahonen; A Linnala-Kankkunen; P H Mäenpää

doi:10.1016/0014-5793(88)80581-7

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Selective decrease in low-Mr HMG proteins HMG I and HMG Y during differentiation of mouse teratocarcinoma cells.

E Vartiainen·J Palvimo·A Mahonen·A Linnala-Kankkunen·P H Mäenpää

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Abstract

We have studied the presence of high-mobility-group (HMG) chromatin proteins in undifferentiated F9 mouse teratocarcinoma cells and F9 cells, which were induced to differentiate by treatment with retinoic acid and dibutyryl-cAMP for 5 days. Acetic acid/urea-polyacrylamide gel electrophoresis and reversed-phase HPLC revealed that the induced F9 cells contained 77 and 62% less HMG I and HMG Y, respectively, than their untreated counterparts. The relative amounts of two other low-Mr HMG proteins HMG 14 and HMG 17 remained essentially unchanged and only a minor decrease was observed in the content of one of the high-Mr HMG proteins, HMG 2. The identity of the low-Mr HMG proteins was verified by amino acid analysis or partial sequencing. These results suggest that HMG I and HMG Y are HMG proteins specific for undifferentiated cells.

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Amino Acid Sequence Animals Cattle Cell Differentiation Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel High Mobility Group Proteins Mice Solubility Teratoma Thermolysin Thymus Gland Journal Article Research Support, Non-U.S. Gov't

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E Vartiainen, J Palvimo, A Mahonen, A Linnala-Kankkunen, P H Mäenpää. 1988-02-08. Selective decrease in low-Mr HMG proteins HMG I and HMG Y during differentiation of mouse teratocarcinoma cells.. https://doi.org/10.1016/0014-5793(88)80581-7

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