Crosslinked insulins: preparation, properties, and application.

D Brandenburg; H G Gattner; W Schermutzki; A Schüttler; J Uschkoreit; J Weimann; A Wollmer

doi:10.1007/978-1-4684-3282-4_16

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Crosslinked insulins: preparation, properties, and application.

D Brandenburg·H G Gattner·W Schermutzki·A Schüttler·J Uschkoreit·J Weimann·A Wollmer

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Abstract

Crosslinked insulins have proved to be valuable for structure-function studies and as proinsulin models. In the first part of the paper, a short review of the literature on analytical investigations, the preparation of A1-B1- and A1-B29-crosslinked derivatives, their biological activities in vivo and in vitro, and CD-spectral properties is given. The results of reduction/reoxidation studies with insulin derivatives containing irreversible and cleavable crosslinks are summarized. In the second part, new A1-B29-crosslinked monomers and 3 symmetrical dimers, linked between A1-A'1, B1-B'1 and B29-B'29, are described, as well as some results of tritium-labelling and of enzymatic degradation experiments with A1-B29-linked insulins.

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Amino Acid Sequence Chemical Phenomena Chemistry Disulfides Insulin Isotope Labeling Macromolecular Substances Methods Models, Molecular Oxidation-Reduction Proinsulin Structure-Activity Relationship Tritium Journal Article Review

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D Brandenburg, H G Gattner, W Schermutzki, A Schüttler, J Uschkoreit, J Weimann, A Wollmer. 1977. Crosslinked insulins: preparation, properties, and application.. https://doi.org/10.1007/978-1-4684-3282-4_16

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