PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 33819

Experimental hypoplastic marrow failure in the mouse.

K Kubota·H Mizoguchi·Y Miura·S Kano·F Takaku
View original source ↗Export metadata ↓

Abstract

In order to study the pathogenesis of aplastic anemia in man, hemopoietic stem cells were investigated in 'aplastic mice' the aplasia being induced by the immunological method. C3H/He (H-2k, Mlsc) received 600 rad whole body x-irradiation followed by the transplantation of 10(7) lymph node cells prepared from B10.BR mic e (H-2k, Mlsb). The C3H/He mice developed pancytopenia and marrow hypoplasia 21 days after these treatments. The total number of nucleated cells, CFU-S and CFU-C in the marrow and the wet weight and CFU-C of the spleen were markedly reduced. These findings are consistent with those of aplastic anemia in man and the model may provide a useful tool for the investigation of the pathogenesis of this anemia. Control mice that received irradiation only recovered from the damage 21 days later, while control mice that receive lymph node cells only showed no hematological changes.

Explore related subjects

Anemia, AplasticAnimalsBlood PlateletsBody WeightBone MarrowCell SurvivalColony-Forming Units AssayFemaleGraft vs Host ReactionHematocritHematopoietic Stem CellsHistocompatibility AntigensLymph NodesMiceModels, BiologicalOrgan SizeRadiation Injuries, ExperimentalSpleenTransplantation, HomologousJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

K Kubota, H Mizoguchi, Y Miura, S Kano, F Takaku. 1978. Experimental hypoplastic marrow failure in the mouse.. https://pubmed.ncbi.nlm.nih.gov/33819/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1979

Association between pre-transplant natural kill and graft-versus-host disease after stem-cell transplantation.

Natural killer activity against herpes simplex virus type 1 infected fibroblasts NK(HSV-1) was studied prospectively in patients undergoing allogenic bone-marrow or fetal-tissue stem-cell transplantation. Thirteen patients showed evidence of engraftment and survived long enough to develop graft-versus-host disease (GvHD). Of this group, all of the seven having normal NK(HSV-1) activity before transplantation acquired GvHD and the six having low NK(HSV-1) had no evidence of GvHD. These results were independent of mode of preparation of patients for transplantation, source of stem cells used, or cytomegalovirus infections, and they suggest that this assay reflects a host-determined function capable of stimulating GvHD.

Anemia, Aplastic↗
PubMed1979

Major histocompatibility complex-restricted antibody reactivity mainly, but not exclusively, directed against cells from male donors.

An IgM antibody, present in the serum of a female patient with aplastic anemia, is described that reacted in a modified complement-dependent cytotoxicity test with a subset of the B cells from HLA-A2-positive, but not HLA-A2-negative males. With the exception of two HLA-A2 positive females, the antibody did not react with other cells from either HLA-A2-positive or HLA-2-negative females. The cells of one of these and from HLA-A2-positive males were able to absorb the antibody from the serum. Cells from other donors were unable to absorb the antibodies. The mononuclear cells of the same patient were cytolytic in cell-mediated lympholysis (CML) for phytohemagglutinin blasts from all HLA-A2-positive males and one of the females reacting with antibody, but not with blasts from HLA-A2-negative males and all other females. Thus, the results obtained with the antibody in the complement-dependent cytotoxicity test showed an almost perfect correlation with cytolysis in CML tests. These results suggest that the IgM antibody may be the first example with major histocompatibility complex restriction. Because the antibody reacted with the cells from two female donors, the restricting determinant is not, in all probability, the H-Y determinant.

Anemia, Aplastic↗