Processivity of DNA exonucleases.

K R Thomas; B M Olivera

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PubMed · 338608

Processivity of DNA exonucleases.

K R Thomas·B M Olivera

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Abstract

A homopolymer system has been developed to examine the digestion strategies of DNA exonucleases. Escherichia coli exonuclease I and lambda-exonuclease, are processive enzymes. However, T7 exonuclease, spleen exonuclease, E. coli exonuclease III, the 3' leads to 5'-exonuclease of T4 DNA polymerase, and both the 3' leads to 5' and the 5' leads to 3' activity of E. coli DNA polymerase I dissociate frequently from the substrate during the course of digestion. Regions of duplex DNA are a dissociation signal for exonuclease I.

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Animals Coliphages Deoxyribonucleases Escherichia coli Exonucleases Kinetics Molecular Weight Spleen Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K R Thomas, B M Olivera. 1978-01-25. Processivity of DNA exonucleases.. https://pubmed.ncbi.nlm.nih.gov/338608/

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