Computerized image-analysis microspectroscopy of tissue sections.

M A Pappolla

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PubMed · 3395214

Computerized image-analysis microspectroscopy of tissue sections.

M A Pappolla

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Abstract

A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.

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Animals Cerebellum Culture Techniques Image Processing, Computer-Assisted Microscopy Rats Spectrum Analysis Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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M A Pappolla. 1988. Computerized image-analysis microspectroscopy of tissue sections.. https://pubmed.ncbi.nlm.nih.gov/3395214/

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