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Quantitation of polyamines using thin-layer chromatography and image analysis.

S H Wettlaufer·L H Weinstein
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Abstract

Efficient separation of dansylated polyamines can be achieved by thin-layer chromatography (TLC). Quantitation, however, can be laborious because it requires removal of the silica gel and the fluorescing derivative from the glass plates, elution in a suitable solvent, and estimation with a fluorescence spectrophotometer. We report here a relatively simple and rapid method for the quantitation of dansylated polyamines that employs an image analyzer without removal from the glass TLC plates.

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Chromatography, Thin LayerPlantsPolyaminesPutrescineSpectrophotometry, UltravioletSpermidineSpermineJournal Article

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S H Wettlaufer, L H Weinstein. 1988-06-10. Quantitation of polyamines using thin-layer chromatography and image analysis.. https://doi.org/10.1016/s0021-9673(01)83879-6

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