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PubMed · 3413061

A repressor heterodimer binds to a chimeric operator.

M Hollis·D Valenzuela·D Pioli·R Wharton·M Ptashne
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Abstract

Replacement of the solvent-exposed residues of the DNA recognition helix of the 434 repressor with the corresponding residues of the P22 repressor generates a hybrid protein, 434R[alpha 3(P22R)], which binds specifically to P22 operators. We show here that a new DNA-binding specificity is generated by combining 434 and 434R[alpha 3(P22R)] repressor monomers to form a heterodimer. The heterodimer specifically recognizes a chimeric P22/434 operator that lacks two-fold rotational symmetry.

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Base SequenceDeoxyribonuclease IMutationOperator Regions, GeneticRepressor ProteinsTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M Hollis, D Valenzuela, D Pioli, R Wharton, M Ptashne. 1988. A repressor heterodimer binds to a chimeric operator.. https://doi.org/10.1073/pnas.85.16.5834

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