Expressed recombinant cadherins mediate cell sorting in model systems.

A Nose; A Nagafuchi; M Takeichi

doi:10.1016/0092-8674(88)90114-6

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Expressed recombinant cadherins mediate cell sorting in model systems.

A Nose·A Nagafuchi·M Takeichi

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Abstract

Cadherins are cell-surface glycoproteins responsible for Ca2+-dependent cell-to-cell adhesion. E- or P-cadherin was transfected into L cells, which normally have little cadherin activity, and cellular aggregation of the resulting transfectants was observed to be a function of the cadherin molecule expressed. Transfected cells preferentially adhered to cells expressing the same cadherin subclass. Furthermore, in reconstituted embryonic lung tissue, E-cadherin-expressing L cells were associated with epithelial tubules expressing E-cadherin, while untransfected L cells associated with mesenchymal cells. These results provide the first direct evidence that the differential expression of cadherins can play a role in cell sorting in heterogeneous cell populations.

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Agglutination Animals Antigens, Surface Cell Adhesion Cell Adhesion Molecules Cell Aggregation Genetic Vectors L Cells Lung Mice Mice, Inbred ICR Models, Genetic Protein Binding Recombinant Proteins Time Factors Transfection Journal Article Research Support, Non-U.S. Gov't

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A Nose, A Nagafuchi, M Takeichi. 1988-09-23. Expressed recombinant cadherins mediate cell sorting in model systems.. https://doi.org/10.1016/0092-8674(88)90114-6

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