Whole embryo morphometry in teratogen screening.

Abstract

Current methodology in embryo evaluation involves qualitative assessment of razor blade and paraffin serial sections. Presently, no one has applied existing computerized morphometric techniques to examine embryos. A technique has been developed that enables investigators to section embryos at 150 mu, thereby greatly reducing the number of sections and making morphometric analysis possible. This type of analysis permits the precise volumetric determination of several developing organ systems. The aim of this study was to evaluate the feasibility and sensitivity of whole embryo morphometry in teratogen screening. Cadmium chloride, a well-established teratogen, was chosen because of its ability to induce exencephaly in approximately one-half of offspring while having no observable effects on the remaining exposed embryos. It was found that both exencephalic and normal-appearing cadmium-exposed embryos had significantly smaller total cellular, neuroepithelial, otic vesicle, optic assembly, limb bud, and cardiac mesenchyme volumes when compared to controls. Also, the neuroepithelial volume of the exencephalic embryos was significantly smaller than the normal-appearing cadmium-exposed embryos. These results suggest that in addition to inducing exencephaly, cadmium chloride has an overall inhibitory effect on embryonic growth. We have shown that whole embryo morphometry is a sensitive means of evaluating embryonic growth that permitted determination of cadmium-induced aberrations not discernable by currently employed techniques. In light of these results, we feel this technique shows promise for future investigations of known and suspected teratogens.