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A rapid, simple method for isolation of viable microfilariae.

M Van Hoegaerden·B Ivanoff
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Abstract

We describe a rapid, efficient method for purifying Loa loa microfilariae from blood that does not affect their longterm motility. It is based on the separation of microfilariae on a performed discontinuous Percoll gradient, followed by filtration on cellulose ester membranes. The parasite population, after isolation, is close to 100% pure, 100% viable and remains motile for at least 20 days. Recovery from original blood samples is typically around 90%. The method, because of its simplicity and minimal material requirements, could be used with up to 210 ml of blood. It also allows the isolation of Mansonella perstans microfilariae.

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AnimalsCentrifugation, IsopycnicFilariasisHumansLoaLoiasisMansonellaMansonelliasisJournal Article

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BibTeXRIS

M Van Hoegaerden, B Ivanoff. 1986. A rapid, simple method for isolation of viable microfilariae.. https://doi.org/10.4269/ajtmh.1986.35.148

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