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Human recipient cell for oncogene transfection studies.

M A Tainsky·F L Shamanski·D Blair·G Vande Woude
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Abstract

We used human oncogene DNA to transform the nontumorigenic, revertant, human osteosarcoma cell line HOS TE-85 clone 5 (ATCC CRL 1543) to tumorigenicity in athymic nude mice with latency periods as short as 3 weeks. These cells were also transformed by genetic markers in genomic DNA samples. Because of their low rate of spontaneous tumor formation and the simplicity of culturing them, HOS cells provide a human cell alternative to NIH 3T3 murine fibroblasts for oncogene transfection studies.

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AnimalsCell LineCell Transformation, NeoplasticDNAHumansMiceMice, NudeNeoplasm TransplantationOncogenesOsteosarcomaProto-OncogenesTransfectionJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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M A Tainsky, F L Shamanski, D Blair, G Vande Woude. 1987. Human recipient cell for oncogene transfection studies.. https://doi.org/10.1128/mcb.7.3.1280-1284.1987

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