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Amplified ribosomal spacer sequence: structure and evolutionary origin.

R Kominami·M Muramatsu
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Abstract

A novel class of repeated sequences consisting of tandem arrays of ribosomal spacer sequence has been discovered in a mouse genome. Comparison to normal ribosomal DNA reveals that one repeat unit consists of two separate parts of spacer sequence. This amplified spacer sequence has a pseudogene-like structure but is distinct from the previously reported pseudogenes and orphons in regions lacking coding sequences. So far the amplified spacer sequence has been found only in the BALB/c mouse genome but not in ten other laboratory strains and several wild-type mouse stocks. Surprisingly, a part of the amplified spacer sequence unit had a higher homology to the corresponding part of the ribosomal DNA sequence of Mus musculus molossinus, a Japanese wild-type mouse, than to the corresponding part of the rDNA of the BALB/c mouse. These findings suggest that the amplified spacer sequence of the BALB/c mouse might have partly originated in M. m. molossinus or in a related subspecies.

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AnimalsBase SequenceDNA, RecombinantDNA, RibosomalMiceMice, Inbred BALB CNucleic Acid HybridizationRNA, RibosomalJournal ArticleResearch Support, Non-U.S. Gov't

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R Kominami, M Muramatsu. 1987-01-05. Amplified ribosomal spacer sequence: structure and evolutionary origin.. https://doi.org/10.1016/0022-2836(87)90639-5

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