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A joining-diversity-joining complex generated by inversion mechanism and a variable-diversity complex in the beta-chain gene of the human T-cell receptor.

K Ikuta·T Ogura·A Shimizu·T Honjo
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Abstract

We have analysed an inactive allele of the beta-chain gene of the T-cell receptor in a human T-cell line HPB-ALL. Comparison with germline sequences showed that HPB-ALL has a joining (J)-diversity (D)-J complex recombined in head-to-head configuration and a variable (V)-D complex in tail-to-tail configuration. These results demonstrate that the inversion mechanism functions in the beta-chain gene of the T-cell receptor. The presence of the V-D complex suggests that V-D recombination could occur prior to D-J recombination although there is no definite proof that the V-D complex is an intermediate to form the V-D-J complex.

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Base SequenceCell DifferentiationCell LineChromosome InversionCloning, MolecularGenesHumansReceptors, Antigen, T-CellRecombination, GeneticT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov't

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K Ikuta, T Ogura, A Shimizu, T Honjo. 1986-06-25. A joining-diversity-joining complex generated by inversion mechanism and a variable-diversity complex in the beta-chain gene of the human T-cell receptor.. https://doi.org/10.1093/nar%2F14.12.4899

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