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Genes coding for the elongation factor EF-1 alpha in Artemia.

J A Lenstra·A Van Vliet·A C Arnberg·F J Van Hemert·W Möller
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Abstract

The elongation factor EF-1 alpha is one of the most abundant proteins in eukaryotic cells, where it catalyzes the binding of aminoacyl-tRNA to ribosomes. The genes coding for this protein in the brine shrimp Artemia were analyzed by gene cloning, electron microscopy and chromosomal blot hybridization. There are only a few (about four) copies of one type of gene per haploid genome. These genes contain five exons divided over 10(4) base pairs. Local rearrangements give rise to a number of gene variants. Cross-hybridizations of Artemia cDNA probes with yeast and Drosophila DNA revealed two different yeast EF-1 alpha genes and one or two different Drosophila genes, respectively. Nucleotide sequencing revealed signals for synthesis and processing of EF-1 alpha transcripts as well as the exact location of exons. One interruption in the coding sequence corresponds closely to a splice junction in the gene coding for the homologous chloroplast protein EF-Tu from Euglena gracilis, presumably of prokaryotic origin. The first exon in the chloroplast gene codes for the region of EF-Tu that is homologous to regions of the elongation factor EF-G and of the initiation factor IF2, respectively.

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AnimalsArtemiaBase SequenceCloning, MolecularDNADrosophilaGenesMicroscopy, ElectronNucleic Acid HybridizationPeptide Elongation Factor 1Peptide Elongation FactorsProtein BiosynthesisSaccharomyces cerevisiaeJournal ArticleResearch Support, Non-U.S. Gov't

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J A Lenstra, A Van Vliet, A C Arnberg, F J Van Hemert, W Möller. 1986-03-17. Genes coding for the elongation factor EF-1 alpha in Artemia.. https://doi.org/10.1111/j.1432-1033.1986.tb09514.x

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