Interaction between actin and HMM.

J Borejdo; M Giordano

doi:10.1016/s0006-291x(86)80207-8

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Interaction between actin and HMM.

J Borejdo·M Giordano

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Abstract

It is shown that the interaction between actin and HMM results in a rapid precipitation of acto-HMM gel upon addition of MgATP. This is a simple demonstration of the idea that the formation of myosin filaments is not essential for mechanochemical reaction (muscle contraction) to occur and that the soluble myosin heads are competent to interact with actin to produce mechanical effect. Our findings also strongly support earlier suggestion that each head of one HMM molecule is able to bind to a different actin filament.

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Actins Adenosine Triphosphate Animals Gels In Vitro Techniques Muscle Contraction Myosin Subfragments Myosins Osmolar Concentration Peptide Fragments Protein Binding Spectrum Analysis Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J Borejdo, M Giordano. 1986-12-15. Interaction between actin and HMM.. https://doi.org/10.1016/s0006-291x(86)80207-8

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