PubMed · 3545924
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P Claney. Alginate impression procedures.. https://pubmed.ncbi.nlm.nih.gov/3545924/
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The increased wearing of gloves during dental treatment has created new problems for general dental practitioners. One of these is the retarded set or total inhibition of addition silicones caused by natural latex gloves. This study was designed to test different gloves and impression materials in common use. Eight latex gloves, selected with different properties representing all possible types, together with four non-latex gloves, made of neoprene, styrene-butadiene, polyethylene and polyvinylchloride, were tested. As a control, mixing with bare hands and with maize starch was carried out. The viscosity changes of five types of impression materials (polyvinylsiloxanes, condensation silicones, alginates, polyether and polysulphides) were measured with a Zwick hardness tester to give the Shore A hardness. No impression materials other than polyvinylsiloxanes were affected by pure latex gloves and those only by two brands. All other impression materials can be mixed and handled with gloved hands without affecting their setting time. The retarded set of polyvinylsiloxanes caused by natural latex gloves is not so widespread as had previously been thought.
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Chronic infection by alginate-producing (mucoid) Pseudomonas aeruginosa is the leading cause of mortality among cystic fibrosis (CF) patients. During the course of sustained infection, the production of an alginate capsule protects the bacteria and allows them to persist in the CF lung. One of the key regulators of alginate synthesis is the algT (algU) gene encoding a putative alternative sigma factor (sigma E). AlgT was hyperproduced and purified from Escherichia coli. The N-terminal sequence of the purified protein matched perfectly with that predicted from the DNA sequence. The purified protein, in the presence of E. coli RNA polymerase core enzyme, was able to initiate transcription of an algT promoter. Deletion of the -35 region of this promoter abolished this activity in vitro as well as in vivo. These data indicate that the algT gene encodes a sigma factor that is autoregulatory.