Decrease in the levels of a constitutive cytochrome P-450 (RLM5) in hepatic microsomes of diabetic rats.

L V Favreau; J B Schenkman

doi:10.1016/0006-291x(87)91460-4

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Decrease in the levels of a constitutive cytochrome P-450 (RLM5) in hepatic microsomes of diabetic rats.

L V Favreau·J B Schenkman

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Abstract

Cytochrome P-450 dependent hydroxylation of testosterone has been measured in hepatic microsomes of control, diabetic and insulin-treated diabetic rats. The observed decrease in testosterone 16 alpha-hydroxylase activity in diabetes, an activity previously shown to be largely due to RLM5, was accompanied by a dramatic decrease in immunodetectable RLM5. Diabetic rats which received insulin had elevated testosterone 16 alpha-hydroxylase activity relative to the diabetic animals, which was accompanied by a corresponding increase in the levels of RLM5. These results provide evidence that specific constitutive cytochrome P-450 enzymes are altered in the diabetic state and that these changes are not permanent since they can be overcome, at least partially, by insulin replacement therapy.

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Animals Aryl Hydrocarbon Hydroxylases Cytochrome P-450 Enzyme System Diabetes Mellitus, Experimental Insulin Male Microsomes, Liver Rats Rats, Inbred Strains Steroid 16-alpha-Hydroxylase Steroid Hydroxylases Journal Article Research Support, U.S. Gov't, P.H.S.

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L V Favreau, J B Schenkman. 1987-02-13. Decrease in the levels of a constitutive cytochrome P-450 (RLM5) in hepatic microsomes of diabetic rats.. https://doi.org/10.1016/0006-291x(87)91460-4

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