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PubMed · 3549456

A shuttle vector system for Brevibacterium lactofermentum.

P Yeh·J Oreglia·F Prévots·A M Sicard
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Abstract

We have constructed a shuttle vector that replicates in Escherichia coli, Corynebacterium glutamicum and Brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from B. lactofermentum and a derivative of pBR322. Resistance to erythromycin which is expressed in all three bacteria has been a useful selective marker. The frequency of homospecific transformation was 1.5 X 10(5) transformants/micrograms of hybrid plasmid DNA.

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BrevibacteriumCorynebacteriumDNA, BacterialErythromycinEscherichia coliGenetic VectorsPlasmidsR FactorsTransformation, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

P Yeh, J Oreglia, F Prévots, A M Sicard. 1986. A shuttle vector system for Brevibacterium lactofermentum.. https://doi.org/10.1016/0378-1119(86)90074-0

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