Detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates.

S S Yang; R D Coleman

doi:10.1016/0003-2697(87)90079-0

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Detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates.

S S Yang·R D Coleman

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Abstract

After electrophoresis, active pullulanase bands in acrylamide gels have been detected by overlaying and then incubating the gel on a replica gel containing 2.5% pullulan-reactive red conjugate and 2.1% agar. The enzyme activity is revealed as a clear band against a red background on the replica gel. The sensitivity of the replica plate is such that 0.0012 unit of Klebsiella aerogenes pullulanase can be detected easily. This procedure is effective in enzyme screening to distinguish pullulanase from other carbohydrases.

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Agar Electrophoresis, Polyacrylamide Gel Glucans Glycoside Hydrolases Klebsiella pneumoniae Journal Article

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S S Yang, R D Coleman. 1987-02-01. Detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates.. https://doi.org/10.1016/0003-2697(87)90079-0

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