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Pleiotropic changes in cycloheximide-resistant insect cell clones.

N Nouri·A M Fallon
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Abstract

Somatic cell mutants resistant to drugs that interact with the eukaryotic ribosome provide a useful tool for studies on ribosome structure, function, and genetics. From Aedes albopictus (mosquito) cells, cycloheximide-resistant mutants (Cx-705 and Cx-738) that were about 30-fold more resistant to cycloheximide than the parental cells have been obtained. The observation that protein synthesis in cell-free lysates from Cx-705 and Cx-738 cells was resistant to cycloheximide led us to suspect that the alteration in these mutants might affect the ribosome. The present studies show that the cycloheximide-resistant cells grow poorly and eventually die at 34.5 degrees C, a temperature at which wild-type cells grow normally. Relative to control cells, the cycloheximide-resistant cells show increased sensitivity to G-418, another antibiotic that interacts with the eukaryotic ribosome. However, there were no differences between cycloheximide-resistant cells and wild-type cells in sensitivity to puromycin, emetine, or cryptopleurine. Cx-705 cells were predominantly diploid; in contrast, the frequency of tetraploid nuclei in Cx-738 cells was about 40%.

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AedesAnimalsAnti-Bacterial AgentsCell DivisionCentrifugation, Density GradientClone CellsCycloheximideDrug ResistanceGentamicinsKaryotypingMutationRibosomal ProteinsTemperatureJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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N Nouri, A M Fallon. 1987. Pleiotropic changes in cycloheximide-resistant insect cell clones.. https://doi.org/10.1007/bf02623577

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