PubMed · 3566009
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L Boniforti, M G Quaglia, M Terracciano. 1987. [Pharmaceutical and toxicologic analysis].. https://pubmed.ncbi.nlm.nih.gov/3566009/
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Membrane adsorbers are well established in protein chromatography. The present paper investigated for the first time the behavior of polynucleotides on these stationary phases, taking a 7.2-kb predominantly supercoiled plasmid as example. Gradient and isocratic elution was studied. In contrast to protein high-performance membrane chromatography (HPMC), isocratic elution is possible in DNA chromatography. In the case of gradient elution, much higher salt concentrations can be used in the starting buffer. Under optimized conditions, both approaches led to a splitting of the single plasmid peak into three maximums, which corresponded to the three-albeit isolated-bands in the agarose gel. Presumably the three fractions were supercoiled, nicked, and open circular plasmid DNA. Linearization of the plasmid lowered the adsorption energy, and the linearized plasmid eluted earlier than the nonlinearized one. The HPMC experiments were compared to similar ones performed using a conventional packed-bed anion-exchange column (BioScale Q2, 7 x 52 mm, 10-micron porous particles) and a novel monolithic-type anion-exchange column (UNO Q1, 7 x 35 mm). The results and characteristic differences observed in these experiments were interpreted in the light of the newly developed theory of HPMC.
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This paper highlights the status of thin-layer chromatography (TLC) of mycotoxins in various sample matrices. The outstanding merits of TLC in the field of the qualitative and quantitative determination of mycotoxins have been briefed. A comparison between different TLC methods and TLC with HPLC, enzyme-linked immunosorbent assay and GC methods, etc. is made, in general.