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A sensitive haemolytic assay of mouse complement.

J H Berden·J F Hagemann·R A Koene
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Abstract

Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 30 degrees C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml.

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AnimalsComplement System ProteinsHemolytic Plaque TechniqueMethodsMiceOsmolar ConcentrationTemperatureTime FactorsJournal Article

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J H Berden, J F Hagemann, R A Koene. 1978. A sensitive haemolytic assay of mouse complement.. https://doi.org/10.1016/0022-1759(78)90118-7

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