Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

A C Chang; J H Nunberg; R J Kaufman; H A Erlich; R T Schimke; S N Cohen

doi:10.1038/275617a0

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Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

A C Chang·J H Nunberg·R J Kaufman·H A Erlich·R T Schimke·S N Cohen

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Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

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Animals Base Sequence DNA, Recombinant Escherichia coli Genes Mice Phenotype Plasmids Tetrahydrofolate Dehydrogenase Trimethoprim Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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A C Chang, J H Nunberg, R J Kaufman, H A Erlich, R T Schimke, S N Cohen. 1978-10-19. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.. https://doi.org/10.1038/275617a0

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