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cDNA clones encoding bovine gamma-crystallins.

R E Hay·W D Woods·R L Church·J M Petrash
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Abstract

We have determined the nucleotide sequence of two bovine lens gamma-crystallin cDNA clones, pBL gamma II-1 and pBL gamma III-1. The 644 bp cDNA insert of pBL gamma II-1 contains coding information for the entire amino acid sequence of bovine gamma II-crystallin. The 497 bp cDNA insert of pBL gamma III-1 encodes a homologous but different gamma-crystallin polypeptide, and appears to lack the coding information for the C-terminal 17 amino acid residues. While the nucleotide and predicted amino acid sequences of the coding regions of the clones show a high degree of homology, the untranslated leader sequences are relatively dissimilar. The leader sequence of pBL gamma III-1 is strikingly homologous to a portion of a rabbit immunoglobulin alpha-heavy chain mRNA.

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Amino Acid SequenceAnimalsBase SequenceCattleCloning, MolecularCodonCrystallinsDNAHumansMiceRatsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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R E Hay, W D Woods, R L Church, J M Petrash. 1987-07-15. cDNA clones encoding bovine gamma-crystallins.. https://doi.org/10.1016/0006-291x(87)90729-7

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