A general transcription factor forms a stable complex with RNA polymerase B (II).

X M Zheng; V Moncollin; J M Egly; P Chambon

doi:10.1016/0092-8674(87)90490-9

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A general transcription factor forms a stable complex with RNA polymerase B (II).

X M Zheng·V Moncollin·J M Egly·P Chambon

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Abstract

A general transcription factor (BTF3) has been purified from HeLa whole cell extracts and shown to be required for accurate initiation of transcription from the adenovirus-2 major late promoter (Ad2MLP) and other RNA polymerase class B promoters. We show that purified BTF3 (27 kd) binds to RNA polymerase B (II), forming a complex that is transcriptionally active. We found no evidence that purified BTF3 interacts with DNA or is required for the formation of the stable preinitiation complex.

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Adenoviridae DNA-Directed RNA Polymerases HeLa Cells Humans Promoter Regions, Genetic Transcription Factors Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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X M Zheng, V Moncollin, J M Egly, P Chambon. 1987-07-31. A general transcription factor forms a stable complex with RNA polymerase B (II).. https://doi.org/10.1016/0092-8674(87)90490-9

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