The H-2Kkml mutation: nucleotide sequence and comparative analysis.

J M Martinko; J C Solheim; J Geliebter

doi:10.1016/0161-5890(87)90092-7

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The H-2Kkml mutation: nucleotide sequence and comparative analysis.

J M Martinko·J C Solheim·J Geliebter

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Abstract

Nucleotide sequence analysis of mRNA from the class I murine MHC mutant H-2Kkml has established a site of mutation to be at the codon for amino acid position 152. Complete sequence information for the nucleotides coding for amino acids 136-163 demonstrates an A----C alteration at the codon for amino acid 152, changing Asp (GAT) in Kk to Ala (GCT) in Kkml. Several other murine and human class I MHC variants have similar alterations at amino acid position 152, resulting in altered biological activity. Finally, the pH-2III pseudogene of the H-2k haplotype has a GCT codon at amino acid position 152, suggesting that the GCT codon occurring in Kkml is the result of a gene conversion event.

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Animals Base Sequence Electrophoresis, Polyacrylamide Gel H-2 Antigens Mice Mice, Inbred CBA Mutation RNA, Messenger Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J M Martinko, J C Solheim, J Geliebter. 1987. The H-2Kkml mutation: nucleotide sequence and comparative analysis.. https://doi.org/10.1016/0161-5890(87)90092-7

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