Characterization of wheat germ initiation factor eIF-2.

R D Clarke; R S Ranu

doi:10.1007/bf00224950

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Characterization of wheat germ initiation factor eIF-2.

R D Clarke·R S Ranu

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Abstract

The initiation factor eIF-2 that specifically binds Met-tRNAf and GTP in ternary complex (eIF-2. GTP. Met-tRNAf) has been purified to apparent homogeneity from wheat germ ribosomal salt wash. The purified factor exhibits a sedimentation coefficient of 5 X 5S and an aggregate molecular weight of 122,000-daltons for the native protein.

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Centrifugation, Density Gradient Chromatography Eukaryotic Initiation Factor-2 Guanosine Triphosphate Molecular Weight Peptide Initiation Factors Proteins RNA, Transfer, Amino Acyl RNA, Transfer, Met Triticum Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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R D Clarke, R S Ranu. 1987. Characterization of wheat germ initiation factor eIF-2.. https://doi.org/10.1007/bf00224950

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