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Flavin-containing monooxygenase and ascorbic acid deficiency. Qualitative and quantitative differences.

J I Brodfuehrer·V G Zannoni
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Abstract

Ascorbic acid deficiency causes qualitative and quantitative differences in the guinea pig hepatic flavin-containing monooxygenase (FMO). Kinetic studies with purified FMO indicated no significant change in the apparent Km of dimethylaniline or NADPH in ascorbate-supplemented or -deficient animals. Following purification of ascorbate-deficient guinea pig FMO by DEAE-cellulose and blue agarose chromatography, exogenous FAD was required for 15% of the FMO microsomal activity recovered. In contrast, only 5% of the total microsomal enzyme recovered from ascorbate-supplemented animals required exogenous FAD. Furthermore, there was an enhanced sensitivity to time-dependent nonlinearity with the purified ascorbate-deficient guinea pig FMO. The degree of time-dependent nonlinearity was related to the concentration of substrate. Also, purified ascorbate-supplemented guinea pig FMO was stable for 4 weeks at -20 degrees, whereas the ascorbate-deficient enzyme was inactivated. A decrease in the quantity of ascorbate-deficient guinea pig FMO compared to ascorbate-supplemented was indicated by a marked reduction in total FMO activity recovered from blue agarose chromatography and reduced protein staining intensity with SDS-PAGE at 56,000 daltons.

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AnimalsAscorbic AcidAscorbic Acid DeficiencyFlavin-Adenine DinucleotideGuinea PigsKineticsMolecular WeightOxygenasesJournal ArticleResearch Support, Non-U.S. Gov't

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J I Brodfuehrer, V G Zannoni. 1987-10-01. Flavin-containing monooxygenase and ascorbic acid deficiency. Qualitative and quantitative differences.. https://doi.org/10.1016/0006-2952(87)90627-7

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