[Simple method of embedding in paraffin under vacuum].

Abstract

A method of embedding into paraffin under vacuum is proposed. Pieces of tissues are placed into a glass jar containing melted paraffin from which the air is pumped out. The jar is permanently placed into TC-80 thermostate. The air-removing hose is placed inside the thermostate through the opening in its upper wall meant for the control thermometer. The air is pumped out by any vacuum pump with electric drive. The proposed method is simple, provides for a good saturation with paraffin, shortens the time of tissue contact with melted paraffin, and gives thin sections of good quality.