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Insulin-like growth factor gene expression during rat embryonic development.

P Rotwein·K M Pollock·M Watson·J D Milbrandt
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Abstract

The function of insulin-like growth factors I and II (IGF-I and IGF-II) in embryogenesis is unknown. To investigate the ontogeny of IGF gene expression during mammalian development we used a highly sensitive and specific solution-hybridization assay to determine the steady state levels of IGF mRNAs during midgestation in the rat. IGF-I mRNA can be detected as early as day 11 of embryonic development and rises 8.6-fold over the ensuing 48 h. By contrast IGF-II mRNA is relatively constant over days 11-14 of gestation. These observations suggest that both IGFs may play important roles in early fetal development.

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AnimalsCloning, MolecularEmbryonic and Fetal DevelopmentGenesInsulin-Like Growth Factor IInsulin-Like Growth Factor IINucleic Acid HybridizationRatsRats, Inbred StrainsSomatomedinsTranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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P Rotwein, K M Pollock, M Watson, J D Milbrandt. 1987. Insulin-like growth factor gene expression during rat embryonic development.. https://doi.org/10.1210/endo-121-6-2141

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