A sequential, solid-phase assay for biotin in physiologic fluids that correlates with expected biotin status.

D M Mock; D B DuBois

doi:10.1016/0003-2697(86)90092-8

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A sequential, solid-phase assay for biotin in physiologic fluids that correlates with expected biotin status.

D M Mock·D B DuBois

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Abstract

Interest in accurate measurement of biotin concentrations in plasma and urine has been stimulated by recent advances in the understanding of biotin-responsive inborn errors of metabolism and by several reports describing acquired biotin deficiency during parenteral alimentation. This paper presents a biotin assay utilizing radiolabeled avidin in a sequential, solid-phase method; the assay has increased sensitivity compared to previous methods (greater than or equal to 10 fmol/tube), correlates with expected trends in biotin concentrations in blood and urine in a rat model of biotin deficiency, and can utilize commercially available radiolabeled avidin.

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Animals Avidin Biotin Iodine Radioisotopes Radioligand Assay Rats Reference Standards Journal Article Research Support, U.S. Gov't, P.H.S.

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D M Mock, D B DuBois. 1986. A sequential, solid-phase assay for biotin in physiologic fluids that correlates with expected biotin status.. https://doi.org/10.1016/0003-2697(86)90092-8

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